BioSample

Single human 8-cell embryo cDNA library was prepared according to the method described by Tang et al., 2010 [PMID:20203668], but stopped before the fragmentation. Embryo collection was performed in collaboration with Outi Hovatta laboratory at the Department of Clinical Science, Technology and Intervention and all experimental work was done in Juha Kere laboratory at the Department of Biosciences and Nutrition, both from Karolinska Istitutet, Sweden. The prediction of clone sequence for primer design was performed by Shintaro Katayama and was based on RNA-seq data [Tohonen, Katayama et al. unpublished]. The transcript was amplified from cDNA library using Phusion High-Fidelity DNA polymerase. PCR product was cloned into pCR4Blunt-TOPO vector and sequenced at Eurofins Genomics, Germany, using T7 primer. The cloning was performed by Eeva-Mari Jouhilahti