BioSample

Human ovary polyA+ RNA was from Clontech (#636152) was used for cDNA synthesis. The prediction of clone sequence for primer design was performed by Shintaro Katayama and was based on RNA-seq data [PMID:26360614]. The transcript was amplified from cDNA library using HotStarPlus Taq DNA polymerase. PCR product was cloned into pCRII-dual promoter TOPO vector and sequenced at Eurofins Genomics, Germany, using T7 primer. The cloning was performed by Eeva-Mari Jouhilahti. All experimental work was done in Juha Kere laboratory at the Department of Biosciences and Nutrition at Karolinska Istitutet, Sweden.