Metagenomic sequencing of KB-1 microbial consotriu... - BioSample

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Metagenomic sequencing of KB-1 microbial consotrium: KB-1/VC-H2 strain KBVC1

Identifiers: BioSample: SAMN06350849; Sample name: KB-1/VC-H2 KBVC1; SRA: SRS6146440

Organism: Dehalococcoides mccartyi
cellular organisms; Bacteria; Bacillati; Chloroflexota; Dehalococcoidia; Dehalococcoidales; Dehalococcoidaceae; Dehalococcoides

Package: MIGS: cultured bacteria/archaea, water; version 6.0

Attributes

strain	KBVC1
number of replicons	missing
relationship to oxygen	anaerobe
isolation and growth condition	missing
environmental medium	water
latitude and longitude	43.6587 N 79.3955 W
broad-scale environmental context	bioreactor
reference for biomaterial	primary publication
depth	groundwater
local-scale environmental context	anaerobic bioreactor
geographic location	Canada: Toronto
collection date	2013-02

Description

Metagenomic sequencing of KB-1: a mixed microbial consotrium used to enhance bioremediation of chlorinated solvents in subsurface environments. This series of cultures were originally enriched from contaminated sediment from Southern Ontario in 1996. Subsequent transfers in 1997 were amended with vinyl chloride (VC) and 5x electron equivalents of methanol (MeOH). The culture was further enriched using 2% transfers in 2001 and grown on VC and 5x electron equivalents of hydrogen gas. Formally named KB-1/VC-H2 after its lineage, electron acceptor and donor. This culture continues to be maintained batch style in anaerobic minimal medium in the Edwards Laboratory at the University of Toronto.

Keywords: GSC:MIxS;MIGS:6.0