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Abe mouse ES cell

Identifiers
BioSample: SAMN00152792; EST: LIBEST_021037
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
cell typeES
cell lineE14
vectorpSPORT 1 (GibcoBRL)
re_1Sal I
re_2Not I
Description

1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-CAGCGGCCGCTTTTTTTTTTTTTTTTTTV-3']; double-stranded cDNA was ligated to Sal I adaptors [5'-TCGACCCACGCGTCCG-3'], digested with Not I and cloned into the Sal I and Not I sites of pSPORT 1 vector.; Average insert size: 0.7 kb (range 0.2-1.3 kb); Sequence primer: T7 [5'-TAATACGACTCACTATAGGG-3']; Primary library, non-amplified.

Submission
National Institute of Radiological Sciences, Masumi Abe; 2007-04-04
Accession:
SAMN00152792
ID:
152792

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