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The UI-R-DN0 library is a non-normalized Rat Distal Colon library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.
Nucleotide
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