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The library was produced by the point-sink shearer method using the Hydroshear DNA sharing device (GeneMachines). The insert size selection was done by gel electrophoresis. After purification and end-repair, the DNA was ligated into pSMART-HC Kan (Lucigen). Purified plasmids were sent for sequencing using primers at each end of the vector (SL1 and SR2 primers). The vector sequence was then removed from the sequence data.
Nucleotide
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