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cDNA was generated from cytoplasmic RNA using a mixture of random DNA hexamers and oligo(dT). From this pool of cDNA, human chromosome 7-enriched cDNA was isolated by direct cDNA selection using chromosome 7 genomic DNA (cosmids). The resulting direct-selected cDNA was cloned into a plasmid vector using a non-directional uracil DNA glycosylase (UDG)-mediated cloning strategy.
Nucleotide
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