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The UI-R-DN1 library is a normalized Rat Distal Colon library (nRDC) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag GAAGTGCTCC between the Not I cloning site and DT18 stretch. The Rat Distal Colon tissue was provided by Tom Freeman of the Sanger Center.
Nucleotide
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