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UI-R-DY1 is a normalized cDNA library containing the following tissue(s): Rat Cartilage from Femur and Tibia. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CTAATGGACG. The Rat cartilage tissue was provided by Dr Jeff Stevens at the University of Iowa.
Nucleotide
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