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McCarrey Eddy 18 day leptotene and zygotene spermatocytes

Identifiers
BioSample: SAMN00170994; EST: LIBEST_011284
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
sexmale
tissue18-day leptotene and zygotene spermatocytes
lab hostDH10B (phage-resistant)
tissuetestis
vectorpBluescript SK+ (Stratagene)
v_typephagemid (ampicillin resistant)
re_1EcoRI
re_2XhoII
Description

cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences).

Submission
Washington University School of Medicine, McCarrey/Eddy NIEHS Mouse; 2002-09-20
Accession:
SAMN00170994
ID:
170994

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