| collection date | 2011-05-01 |
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| broad-scale environmental context | Host-associated |
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| local-scale environmental context | Human |
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| environmental medium | Digestive system |
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| geographic location | Germany |
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| investigation type | metagenome-assembled genome |
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| isolation source | human gut metagenome |
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| project name | To gain more insight into the interconnection between microbiota, obesity and diet, we analyzed the metagenome of 16 patients following a weight-loss program based on a very low calorie inulin-enriched formula diet. In this longitudinal study we used shotgun sequencing of stool DNA to look at taxonomic and functional changes at different phases of the program. In 2 years we sequenced fecal samples at six time points, the baseline (before intervention, = T0), after diet intervention, (3 months later: T3), after reintroduction phase (T6), and three time points at the maintenance phase i.e. month 12 (T12), month 18 (T18) and month 24 (T24). In parallel, clinical parameters were monitored in order to establish potential relationships between microbiota and obesity-associated-disease particularly hypertension, insulin resistance, fatty liver and the metabolic syndrome. Moreover we analyzed the marker potential of microbial parameters for weight loss maintenance success. |
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| sample name | SRR2155332_bin.103_CONCOCT_v1.1_MAG |
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| ENA-CHECKLIST | ERC000047 |
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| ENA-FIRST-PUBLIC | 2023-01-03 |
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| ENA-LAST-UPDATE | 2023-01-03 |
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| External Id | SAMEA14085604 |
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| INSDC center alias | EBI |
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| INSDC center name | European Bioinformatics Institute |
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| INSDC first public | 2023-01-03T00:33:37Z |
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| INSDC last update | 2023-01-03T00:33:37Z |
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| INSDC status | public |
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| Submitter Id | SRR2155332_bin.103_CONCOCT_v1.1_MAG |
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| assembly quality | Many fragments with little to no review of assembly other than reporting of standard assembly statistics |
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| assembly software | metaspadesv3.10.0 |
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| binning parameters | Default |
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| binning software | CONCOCT v1.1 |
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| broker name | EMG broker account, EMBL-EBI |
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| completeness score | 65.8 |
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| completeness software | CheckM |
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| contamination score | 2.03 |
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| geographic location (latitude) | not provided |
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| geographic location (longitude) | not provided |
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| metagenomic source | human gut metagenome |
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| sample derived from | SAMN03922488 |
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| scientific_name | uncultured Clostridia bacterium |
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| sequencing method | Illumina HiSeq 2500 |
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| taxonomic identity marker | multi-marker approach |
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