CSEQCHL20

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Result Filters

Organism: Gallus gallus (chicken)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae; Phasianinae; Gallus

Attributes

strain	White Leghorn, Hisex
development stage	36
lab host	DH10B
tissue	limbs
vector	pBluescript II KS(+)
v_type	phagemid (ampicillin resisitant)
re_1	EcoRI
re_2	Not1

Description: Modification of pBluescript II KS(+) [Stratagene] vector to accommodate cDNA produced with the T-trimmed protocol (Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3' end DNA sequencing by Glenn Fu, et al. U.S. Patent # 6,387,624). Cut pBluescript II KS(+) with NotI and EcoRI. Ligate in double stranded adaptor containing BsgI and BamHI sites [5'ggccgcgtgcagccccggatccgaaaaaaag] [5'aattctttttttcggatccggggctgcacgc]