| broad-scale environmental context | plant |
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| collection date | 2017-10-17 |
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| environmental medium | rhizosphere |
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| geographic location | United Kingdom |
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| investigation type | metagenome-assembled genome |
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| isolation source | rhizosphere metagenome |
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| project name | The microbiota populating the rhizosphere, the interface between roots and soil, can modulate plant growth, development and health. These microbial communities are not stochastically assembled from the surrounding soil but their composition and function is controlled, at least partially, by the host plant. Here we use the staple cereal barley as a model to gain novel insights into the impact of differential applications of nitrogen, a rate-limiting step for global crop production, on the host genetic control of the rhizosphere microbiota. This library contains shotgun metagenomics sequencing from bulk (quarryfield) soil, a modern elite line (Morex) and two wild genotypes: B1K-12 (referered to as Desert), and B1K-31 (referred to as 'North') |
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| sample name | ERR5385023_bin.7_metawrap_v1.3.0_MAG |
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| ENA-CHECKLIST | ERC000047 |
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| ENA-FIRST-PUBLIC | 2022-08-24 |
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| ENA-LAST-UPDATE | 2022-08-24 |
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| External Id | SAMEA110698782 |
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| INSDC center alias | EMG |
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| INSDC center name | EMG |
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| INSDC first public | 2022-08-24T12:18:13Z |
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| INSDC last update | 2022-08-24T12:18:13Z |
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| INSDC status | public |
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| Submitter Id | ERR5385023_bin.7_metawrap_v1.3.0_MAG |
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| assembly quality | Many fragments with little to no review of assembly other than reporting of standard assembly statistics |
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| assembly software | metaSPAdes v3.15.3 |
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| binning parameters | MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters. |
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| binning software | metawrap v1.3.0 |
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| broker name | EMG broker account, EMBL-EBI |
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| completeness score | 94.58 |
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| completeness software | CheckM |
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| contamination score | 2.54 |
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| geographic location (latitude) | 56.45139 |
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| geographic location (longitude) | 3.074722 |
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| local environmental context | barley |
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| metagenomic source | rhizosphere metagenome |
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| sample derived from | SAMEA8103262 |
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| sequencing method | NextSeq 500 |
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| taxonomic classification | The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Sphingobacteriia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Mucilaginibacter;s__ |
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| taxonomic identity marker | multi-marker approach |
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