| broad-scale environmental context | Human digestive system |
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| collection date | 2018 |
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| environmental medium | faeces |
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| geographic location | China |
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| investigation type | metagenome-assembled genome |
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| isolation source | human feces metagenome |
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| project name | Diabetic peripheral neuropathy (DPN) is one of the serious and long-term complications of diabetes mellitus, which has no modifiable treatment. Here we reported the results of using fecal microbiota transplantation (FMT) from the healthy donors as a therapeutic alternative for patients with DPN. Compared to the patients received placebo, the patients with FMT via mid-gut showed significant alleviation of the function of sensory and motor nerves after 12 weeks, accompanying with improvement of the gut barrier integrity and decrease of proinflammatory cytokines. The metagenomic sequencing data suggested that the gut microbiota in patients after FMT had higher capacity to produce butyrate and decreased the biosynthesis of endotoxin. Thus, our work provides the feasibility of using FMT to treatment for DPN. |
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| sample name | DRR221348_bin.13_MetaWRAP_v1.3_MAG |
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| ENA-CHECKLIST | ERC000047 |
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| ENA-FIRST-PUBLIC | 2023-07-18T16:27:38Z |
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| ENA-LAST-UPDATE | 2023-07-18T16:27:38Z |
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| External Id | SAMEA114096350 |
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| INSDC center alias | EMG |
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| INSDC center name | EMG |
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| INSDC first public | 2023-07-18T16:27:38Z |
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| INSDC last update | 2023-07-18T16:27:38Z |
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| INSDC status | public |
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| Submitter Id | DRR221348_bin.13_MetaWRAP_v1.3_MAG |
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| assembly quality | Many fragments with little to no review of assembly other than reporting of standard assembly statistics |
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| assembly software | metaspades v3.15.3 |
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| binning parameters | MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters. |
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| binning software | MetaWRAP v1.3 |
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| broker name | EMG broker account, EMBL-EBI |
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| completeness score | 100 |
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| completeness software | CheckM |
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| contamination score | 0.0 |
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| geographic location (latitude) | 24.7 |
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| geographic location (longitude) | 113.6 |
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| local environmental context | colon |
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| metagenomic source | human feces metagenome |
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| sample derived from | SAMD00218054 |
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| scientific_name | Sutterella wadsworthensis |
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| sequencing method | Illumina HiSeq 3000 |
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| taxonomic classification | The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Sutterellaceae;g__Sutterella;s__Sutterella wadsworthensis |
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| taxonomic identity marker | multi-marker approach |
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