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Knowles Solter mouse 2 cell

Identifiers
BioSample: SAMN00155461; EST: LIBEST_000862
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainC57BL/6J x DBA/2J F1
tissueembryo
development stage2-cell
lab hostDH10B
tissueembryo
vectorpBluescribe (modified)
v_typephagemid (ampicillin resistant)
re_1MluI
re_2SalI
Description

Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.2 kb.

Submission
Washington University School of MedicineP, Marra M/Mouse EST Project; 1997-05-08
Accession:
SAMN00155461
ID:
155461

Nucleotide

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