RT2

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Result Filters

Organism: Saccharum hybrid cultivar SP80-3280
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Saccharinae; Saccharum; Saccharum officinarum species complex; unclassified Saccharum officinarum complex; Saccharum hybrid cultivar

Attributes

cultivar	SP80-3280
lab host	DH10B
tissue	Root tips(0.3cm-long) from adult plants
vector	pSport1
re_1	SalI
re_2	NotI

Description: An unidirectional cDNA library generated from [Root tips(0.3cm-long) from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public