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NIH_MGC_234

Identifiers
BioSample: SAMN00174556; EST: LIBEST_015208
Organism
Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Attributes
tissueheart, pooled
lab hostDH10B TonA
tissueheart
vectorpExpress-1
v_typeplasmid
re_1EcoRV
re_2NotI
Description

RNA obtained from pooled heart tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.2 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_233) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

Submission
National Cancer Institute / NIH, Daniela S. Gerhard, Ph.D.; 2004-01-20
Accession:
SAMN00174556
ID:
174556

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