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hEx1 (RZPD no. 800)

Identifiers
BioSample: SAMN00173360; EST: LIBEST_013865
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuebrain
development stageembryonic, 14.8 and 15.8 weeks (determined by foot length)
vectorpQE30NST (AF074376)
v_typePlasmid
re_1SalI
re_2NotI
Description

1st strand cDNA was prepared from mRNA obtained from brain material of two feti, 14.8 and 15.8 weeks old, with a Not I - oligo(dT) primer [5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Sal I adaptors, digested with Not I and cloned into the Not I and Sal I sites of the pQE30NST vector. This cDNA expression library represents a subset of clones that were selected for protein expression and has been normalised by oligonucleotide fingerprinting. The library was constructed by Konrad Bussow.

Submission
Max Planck Institute of Molecular Genetics, Bussow K; 2003-06-13
Accession:
SAMN00173360
ID:
173360

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