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1st strand cDNA was prepared from mRNA obtained from brain material of two feti, 14.8 and 15.8 weeks old, with a Not I - oligo(dT) primer [5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Sal I adaptors, digested with Not I and cloned into the Not I and Sal I sites of the pQE30NST vector. This cDNA expression library represents a subset of clones that were selected for protein expression and has been normalised by oligonucleotide fingerprinting. The library was constructed by Konrad Bussow.
Nucleotide
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