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1st strand cDNA was primed with an oligo(dT) primer ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [TGTTGGCCTACTGG], digested and cloned into distinct DraIII sites of the pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Size selection was performed to exclude fragments <1.5 kb. Library was constructed by Sugano et al.(University of Tokyo, Institute of Medical Science). Custom primer used for sequencing: 5' end primer [CTTCTGCTCTAAAAGCTGCG], 3' end primer [CGACCTGCAGCTCGAGCACA]
Nucleotide
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