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RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.6 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_246) and was constructed by Open Biosystems. Note: this is a NIH_MGC library
Nucleotide
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