BioSample

An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized the PB element using Hsp70:piggyBac transposase from a single ammunition element on either the X or third chromosome. We induced transposase expression by immersing bottles in a circulating 37oC water bath for a daily (days 3-10 after egg-laying) 1-h heat shock. We outcrossed the resulting dysgenic males to an isogenic w- strain. New insertions were identified on the basis of a change in eye color (third chromosome ammunition) or the appearance of w+ male progeny (X chromosome ammunition). All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR.