FH UGS01

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Result Filters

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Attributes

strain	C57BL6
sex	Male and Female
tissue	Pooled urogenital sinuses
lab host	BM25.8
tissue	prostate
vector	pTriplEx2
v_type	plasmid (ampicillin resistant)
re_1	SfiI
re_2	SfiI

Description: Library was constructed using Clontechs SMART cDNA library construction kit. 1ug of total RNA from pooled urogenital sinuses was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 18 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled urogenital sinuses from E16 mouse embryos (male and female) by Colin Pritchard.