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The apical portion and the entire pulp of the maxillary and mandibular incisors from 3-4 week old rats were excised. A cDNA library was constructed in the lZAP II vector (Stratagene) by use of poly A+ RNA from these tissues. The phage cDNA library was converted into a Bluescript phagemid cDNA library by in vivo excision by the ExAssist/SOLR system (Stratagene).
Nucleotide
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