Figure 13.. Outline of informatics pipeline for processing and analyzing data from massively parallel sequencing platforms.

Figure 13.Outline of informatics pipeline for processing and analyzing data from massively parallel sequencing platforms.

The data analysis process can be broadly divided into three stages, including base calling, alignment, and interpretation. The variant calling and analysis steps of the procedure are not necessary for the clinical microbiology laboratory to perform. Data collection and analysis involve extensive use of various computational methods for converting raw data into sequence information and the application of bioinformatics techniques for the interpretation of that sequence.

Republished from reference 183 with permission from the publisher.

From: Applications of Clinical Microbial Next-Generation Sequencing

Cover of Applications of Clinical Microbial Next-Generation Sequencing
Applications of Clinical Microbial Next-Generation Sequencing: Report on an American Academy of Microbiology Colloquium held in Washington, DC, in April 2015.
Washington (DC): American Society for Microbiology; 2016.
Copyright 2017 American Academy of Microbiology.

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