Figure 3.. Roche 454 GS FLX sequencing.

Figure 3.Roche 454 GS FLX sequencing.

Template DNA is fragmented, end-repaired, ligated to adapters, and clonally amplified by emulsion PCR. After amplification, the beads are deposited into picotiter-plate wells with sequencing enzymes. The picotiter plate functions as a flow cell where iterative pyrosequencing is performed. A nucleotide-incorporation event results in pyrophosphate (PPi) release and well-localized luminescence. APS, adenosine 5'-phosphosulfate.

Republished from reference 5 with permission from the publisher.

From: Applications of Clinical Microbial Next-Generation Sequencing

Cover of Applications of Clinical Microbial Next-Generation Sequencing
Applications of Clinical Microbial Next-Generation Sequencing: Report on an American Academy of Microbiology Colloquium held in Washington, DC, in April 2015.
Washington (DC): American Society for Microbiology; 2016.
Copyright 2017 American Academy of Microbiology.

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