4NAW,3W1S,1WZ3,4GDK


Conserved Protein Domain Family
Ubl_ATG12
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cd01612: Ubl_ATG12 
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ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12)
Autophagy is an essential intracellular process that targets large protein complexes, bacterial pathogens, and organelles for degradation. The autophagy-related ubiquitin-like (Ubl) proteins such as ATG12 protein have a conserved Ubl fold structure and undergo a unique Ubl conjugation, a process essential for autophagosome formation. ATG12 is conjugated to ATG5 by multistep modifications of the E1-like (ubiquitin activating) enzyme ATG7, and the E2-like (ubiquitin conjugating) enzyme ATG10. The ATG12-ATG5 conjugate facilitates the lipidation of ATG8 and directs its correct subcellular localization. ATG12 is localized at the developing autophagosome.
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Statistics
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PSSM-Id: 340454
Aligned: 50 rows
Threshold Bit Score: 85.6463
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:Atg5 conjugation site [polypeptide binding site]
Evidence:
  • Comment:Atg12-Atg5 conjugation site
  • Comment:Atg12 is conjugated to Atg5 through enzymatic reactions similar to ubiquitination.
  • Comment:The Atg12-Atg5 conjugate functions as an E3-like enzyme of ubiquitin system to promote lipidation.
  • Structure:4GDK; Homo sapiens ATG12-ATG5 conjugate bound, contacts at 4A
  • Structure:3WIS; Saccharomyces Cerevisiae Atg12p-Atg5p conjugate bound, contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                     ## #####  #  ###   
4NAW_A         5 KIDILLKAVGDTPi-MKTKKWAVERTRTIQGLIDFIKKFLKLva------seQLFIYVNQsFAPSPDQEVGTLYECFGs- 76  human
4GDK_A         5 KIDILLKAVGDTPi-MKTKKWAVERTRTIQGLIDFIKKFLKLva------seQLFIYVNQsFAPSPDQEVGTLYECFGs- 76  human
Q7S083        70 KVVIRFKPVGSAPa-LRREQVKVLSTHSFETVVAYLRKTLKVqe------tdSVFLYVNSvFAPALDEVVGNLWRCFKd- 141 Neurospora cras...
CAB66169      46 RVNLRFKAIGRTPl-LRKTVFSINASQRFEKVTRFLKKELGLpm------nsSLVLYVNSsFAPSPDEIVGNLYDNFAi- 117 fission yeast
EPR57800     599 KVHISLSNVGGAAr-LRVSRFKVDGYQRFDTVITFLKKALKRd---------HLYVYVNNfIQPQPDEFVADLFKAFGv- 667 Toxoplasma gond...
XP_002766299  23 RVTVQLYPIGNAPp-LKQCKFAMAGSTPVRVLIKFLEGAVMHsgthtgtqepDVYVYINNfFCPMDDQIMADLCREFGkv 101 Perkinsus marin...
Q6CUD5       106 KVKIRLQPIGAIPq-IQPRVCQISAHQQFLALTRFLCKRLKRk---------HIHCYINNaFAPSLDQNIGDLWTQFKv- 174 Kluyveromyces l...
A5DK05       128 KITIRLVPIGSTRa-ITPKVLQVSSDQTVAVLMRFIAKKLRIst-------dSVYMYIHNtFQPTPDERLGDLYDQFRt- 198 Meyerozyma guil...
Q6BZZ1       117 KCQLKFRPIGGATpsLKQSVYKIAETQQFGVVVKFLRKQLKIkns----qssQIFCYISS-FAPGLDETVGSLYNRYAi- 190 Yarrowia lipoly...
Q6BU30        68 KVTIRFQPIGSTPs-INPRVFKISSNQSISTISKFLIKRLKIks-------nLIYLYIQNsFQPNPDEKLGDLYHLFKt- 138 Debaryomyces ha...

Feature 1                              #
4NAW_A        77 ---------DGKLVLHYCKSQAW 90  human
4GDK_A        77 ---------DGKLVLHYCKSQAW 90  human
Q7S083       142 --------sTNQLNVSYSMTPSF 156 Neurospora crassa OR74A
CAB66169     118 ---------DSHLLINYCINVAF 131 fission yeast
EPR57800     668 ---------GGSLMVSYCYTPAY 681 Toxoplasma gondii GT1
XP_002766299 102 erddeqrikGGSLKLHYSVGPAY 124 Perkinsus marinus ATCC 50983
Q6CUD5       175 ---------NDELIVSYCETVAF 188 Kluyveromyces lactis NRRL Y-1140
A5DK05       199 ---------NQELIFNYCNTVAF 212 Meyerozyma guilliermondii ATCC 6260
Q6BZZ1       191 ---------RGELTISYCLNQAF 204 Yarrowia lipolytica CLIB122
Q6BU30       139 ---------NNELIINYCHSIAF 152 Debaryomyces hansenii CBS767

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