2BUF,2BTY,2AP9


Conserved Protein Domain Family
AAK_NAGK-C
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cd04250: AAK_NAGK-C 
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AAK_NAGK-C: N-Acetyl-L-glutamate kinase - cyclic (NAGK-C) catalyzes the phosphorylation of the gamma-COOH group of N-acetyl-L-glutamate (NAG) by ATP in the second step of arginine biosynthesis found in some bacteria and photosynthetic organisms using the non-acetylated, cyclic route of ornithine biosynthesis. In this pathway, glutamate is first N-acetylated and then phosphorylated by NAGK to give phosphoryl NAG, which is converted to NAG-ornithine. There are two variants of this pathway. In one, typified by the pathway in Thermotoga maritima and Pseudomonas aeruginosa, the acetyl group is recycled by reversible transacetylation from acetylornithine to glutamate. The phosphorylation of NAG by NAGK is feedback inhibited by arginine. In photosynthetic organisms, NAGK is the target of the nitrogen-signaling protein PII. Hexameric formation of NAGK domains appears to be essential to both arginine inhibition and NAGK-PII complex formation. NAGK-C are members of the Amino Acid Kinase Superfamily (AAK).
Links
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Statistics
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PSSM-Id: 239783
Aligned: 53 rows
Threshold Bit Score: 331.778
Created: 15-May-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:N-acetyl-L-glutamate binding site [chemical binding site]
Evidence:
  • Structure:2BUF; Pseudomonas aeruginosa NAGK complexed with NAG and ADP, showing contacts with NAG.
  • Comment:defined by 3.5 Angstrom contacts
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                            ##                    ##   
2BUF_E       13 VLSEALPYIRRFVGKTLVIKYGGNAMeseeLKAGFARDVVLMKaVGINPVVVHGGGPQIGDLLKRLSIESHFIDGMRVTD 92  Pseudomonas aeru...
ZP_00372647  29 ILFEVLSNIPSFVGETFVIKCGGTIIldetLFNTFVHNVVLLKqLGINPVIIHDGEYEINSVLKVLGMDSKFVNGIRLTD 108 Wolbachia endosy...
YP_265890    19 TVEEVKKYLEKYNDEFIIIKCGGSVLvdpkLFKVFIEDVVVLKkLGFNPIIVHGGGKRINSKLLEVNIKSNFINGLRVTD 98  Candidatus Pelag...
AAK80344      7 PIEDPLSKLTQYSGKTFVIKYGGSIMknkkAEEAFIKDVKYLRkLGINIVIVHGGGPEISRWLELSGIESRFVDGLRVTD 86  Clostridium acet...
YP_303082    36 VLSECLPYIQQFSGETFVIKYGGAAMtdrkLAESFAHDIVLLKqLGINPIVVHGGGNKINSFLEKINKKSTFINGLRVTD 115 Ehrlichia canis ...
NP_266956    10 TLTESLKYFLKYRDQTVVIKYGGNAMidekVKESILKDILLLKtVGIKVVLVHGGGPAIGELLEKYEQKSQFVQGLRVTD 89  Lactococcus lact...
ZP_00628292  16 TLSEALPYMQRYSGAVVVVKFGGNAMgddeAMAEFARDIVLMKqVGIHPVVCHGGGPMINDLLGKLGIESRFVRGKRVTT 95  Paracoccus denit...
NP_105609    14 LLSRALPYMQRYEHKTVVVKYGGHAMgdieLGKAFARDIALLKqSGVNPIVVHGGGPQIGAMLTKMGIESKFEGGLRVTD 93  Mesorhizobium lo...
YP_192224    36 ILSSALPFLRRYAGDTIVVKYGGHAMgegkLAHAFGYDISLLKlVGINPIVVHGGGPQISAMLDRLKIRSEFIDGLRVTD 115 Gluconobacter ox...
NP_419102    15 TLAEALPYIQIYDRETVVIKYGGHAMgqedVAKVFAADAVLLKlLGVHPVVVHGGGPQISRMLDKAGVKSTFVDGLRVTD 94  Caulobacter cres...

Feature 1                                                                                       
2BUF_E       93 AATMDVVEMVLgGQVNKDIVNLInr----hgGSAIGLTGKDAELIRAKKltvtrqtp-emtkpeiiDIGHVGEVTGVNVG 167 Pseudomonas aeru...
ZP_00372647 109 RDTMKIIEMALcGSVNKKIVQHIns----agGSAIGLCGKDGNLIKVEKitatlredrsnniekilDMGFIGRPTEINPD 184 Wolbachia endosy...
YP_265890    99 KDTINIVEDVL-IEFNKEIVEALne----ldCKAKRITSKENNIITVVQen--------------kDLGFVGRPTGINKE 159 Candidatus Pelag...
AAK80344     87 EKVIEIVQMVLsGKINKKLSLQFni----dgVNAVGLSGVDNKLIEATKkyvyk-------gnetiDIGYVGKVTKVNSE 155 Clostridium acet...
YP_303082   116 AETLEVVEMVLcGLVNKDITQLInk----agGNAIGLCGKDANLIEAKKvcytykenqsnnvekilDMGFVGEPHEVNTD 191 Ehrlichia canis ...
NP_266956    90 KKTAQLALTALaGKVNKSLVQDIir----lgGNAIGVSGIDGKLIEAKPis--------------eDLDYVGEITAIHPE 151 Lactococcus lact...
ZP_00628292  96 RETVEVVEMVLsGLVNKRIVQAInd----agGRAVGISGKDDDLMVCEPdd--------------pELGFVGRPVEMNVQ 157 Paracoccus denit...
NP_105609    94 QKTVEIVEMVLaGSINKEIVALIna----egEWAIGLCGKDGNMVFAEKarktmidp-dsniervlDLGFVGEPVEVDRT 168 Mesorhizobium lo...
YP_192224   116 KSMVDVIEMVLsGSVNKQVTQLInr----agALAVGISGKDGGLIQARRltrtkrdp-dseiekviDLGFVGQPEKIDPR 190 Gluconobacter ox...
NP_419102    95 EATMEVAEMVLsGAINKEIANWItlagaeadVRGVGLSGKDARLITAEKvtrtkkdp-dsnieqavDLGFVGEPTKVDPQ 173 Caulobacter cres...

Feature 1                                    # ##                                               
2BUF_E      168 LLNMLVKg--DFIPVIAPIGVGs-NGESYNINADLVAGKVAEALKAEKLMLLTNIAGLMDk---qGQVLTGLsteqVNEL 241 Pseudomonas aeru...
ZP_00372647 185 ILFFIEEs--DFIPVIAPIGHGk-DGETYHIDADSTASAIAIAVSASKMIILSDTDEEID-----KIGGRKIsvknLNAS 256 Wolbachia endosy...
YP_265890   160 FLTEIIKa--NKVPVIAPLGLDk-DNQTFNINADTTAGSIAIELKARRLMIISDVEGVLDs---eKKLIPEInskkANEL 233 Candidatus Pelag...
AAK80344    156 FIKELLKg--GQVPVIAPIGCDn-KGNVYNINADYAAAFISSALDAEKLIILTDVEGVYRnindpQSIIHEIdikdVNYY 232 Clostridium acet...
YP_303082   192 LLFFIEEs--DFIPVIAPVCSGe-NNITYNVNADLVAGALANALAAAKLIILTNVSGVTDa---nGNLLSEIsvsdAENL 265 Ehrlichia canis ...
NP_266956   152 IIERINQt--DAVPVIASAGIGl-DGEIYNVNADTAASRIAGALSAEQFILLSDVRGLYGnfpdeESFIDEInltnLEKL 228 Lactococcus lact...
ZP_00628292 158 IIRDLYNa--GLIPVIAPVATGmeDNETFNVNGDTAAGAIAGALRADRLLLLTDVAGVKDa---sGEVLTAMhpdqVRAM 232 Paracoccus denit...
NP_105609   169 LLDLLARs--EMIPVLAPVAPGr-DGHTYNINADTFAGAIAGACKASRLLFLTDVPGVLDk---nKKLIDELtvaeAKAL 242 Mesorhizobium lo...
YP_192224   191 VLYALLGa--GLIPVIAPVGVGe-DGETYNINADTAAGAIAGAVHASRLLMLTDVPGVLDe---nGKLIEELsadeAMRR 264 Gluconobacter ox...
NP_419102   174 LIEALLTsehDYIPVVAPIGVSp-DGDTFNINADTVAGALAGALKAKRMLMLTDIKGVLDg---nGELIREMtieqARAL 249 Caulobacter cres...

Feature 1                                                             
2BUF_E      242 ia-dGTIYGGMLPKIRCALEAVQgGVTSAHIIDGRvpNAVLLEIFTDSGVGTLI 294 Pseudomonas aeruginosa
ZP_00372647 257 idcgKIKGEKFIERLMAYVKMAEeCAGVVHIVDGRi-PNIMLDLFTESNSSISI 309 Wolbachia endosymbiont of Drosophila simulans
YP_265890   234 id-qEVISGGMIPKIKNCLDVASnGVKAVVIIDGRknHSLLFELLSDKGSGTLI 286 Candidatus Pelagibacter ubique HTCC1062
AAK80344    233 ik-eEIIKGGMIPKVQCCASAIEnGTKNVQLIDGRndHCLINDILNYRGTIISY 285 Clostridium acetobutylicum ATCC 824
YP_303082   266 ie-qGVANSGMIPKLQTCIKVVKeGYGSAHIIDGRipHVLLLELFTVHGTGTMV 318 Ehrlichia canis str. Jake
NP_266956   229 vk-eKKITDRMIPKIEAIKYAMFeGLGQAVLLDGRvpHALLLELFTDKGQGTMI 281 Lactococcus lactis subsp. lactis Il1403
ZP_00628292 233 ia-dGTIAGGMIPKTETALKALDeGVRAVVILDGRvpNACLLELFTEHGAGSLI 285 Paracoccus denitrificans PD1222
NP_105609   243 ik-dGTVSGGMIPKVETCIEAIErGVEGVVILNGKtpHAVLLELFTEHGAGTLI 295 Mesorhizobium loti MAFF303099
YP_192224   265 ia-dGSISGGMIPKVETCLEAVRkGAKAAVILDGRvpHSCLLELFTRAGPGTLI 317 Gluconobacter oxydans 621H
NP_419102   250 id-tGVATGGMIPKLENAIHAIEsGVEAVVILDGRrpHAMLVELFSEYGAGTLI 302 Caulobacter crescentus CB15

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