GEO DataSets

(Submitter supplied) NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days

Organism:

Haemophilus influenzae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL25730

28 Samples

Download data: TXT

(Submitter supplied) Few studies have investigated host-bacterial interactions at sites of infection in humans using transcriptomics and metabolomics. Haemophilus ducreyi causes cutaneous ulcers in children and the genital ulcer disease chancroid in adults. We developed a human challenge model in which healthy adult volunteers are infected with H. ducreyi on the upper arm until they develop pustules. Here, we characterized host-pathogen interactions in pustules using transcriptomics and metabolomics and examined interactions between the host transcriptome and metabolome using integrated omics. more...

Organism:

Homo sapiens; [Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL31188 GPL32713 GPL24676

20 Samples

Download data: TXT

(Submitter supplied) The transcriptional response of Haemophilus ducreyi to anaerobiosis was determined over the course of 18 h. Cells were collected from aerobic and anaerobic cultures at 4, 8, or 18 hours .

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31188

24 Samples

Download data: TXT

(Submitter supplied) Nontypeable Haemophilus influenzae (NTHi) is a pathobiont which chronically colonises the airway of individuals with chronic respiratory disease. It is unclear how NTHi persists in the airway, however accumulating evidence suggests that NTHi can invade and persist within macrophages. To better understand the mechanisms of NTHi persistence within macrophages, this work developed an in vitro model of NTHi intracellular persistence using human monocyte-derived macrophages (MDM). more...

Organism:

Haemophilus influenzae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL30396 GPL24676

20 Samples

Download data: TXT

(Submitter supplied) Pathogenic bacteria encounter a variety of stressful host environments during infection. Their responses to meet these challenges protect them from deadly damages and aid in adaption to harmful environments. Bacterial products critical for this protection are therefore interesting as suitable targets for new antimicrobials. To shed light on the complex array of molecular pathways involved in bacterial stress responses we challenged 32 diverse human pathogenic bacteria to 11 infection related stress conditions and catalogued their transcriptomes. more...

Organism:

Helicobacter pylori; Enterococcus faecalis; Campylobacter jejuni; Francisella tularensis; Acinetobacter baumannii; Neisseria gonorrhoeae; Escherichia coli; Shigella flexneri; Aggregatibacter actinomycetemcomitans; Haemophilus influenzae; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Neisseria meningitidis; Staphylococcus epidermidis; Streptococcus pyogenes; Listeria monocytogenes; Salmonella enterica; Achromobacter xylosoxidans; Borreliella burgdorferi; Pseudomonas aeruginosa; Legionella pneumophila; Klebsiella pneumoniae; Yersinia pseudotuberculosis; Vibrio cholerae; Streptococcus suis; Streptococcus agalactiae; Streptococcus pneumoniae; Mycobacterium tuberculosis; Burkholderia pseudomallei

Type:

Expression profiling by high throughput sequencing

30 related Platforms

1122 Samples

Download data: TXT

(Submitter supplied) Identify DEGs in H. parainfluenzae in monoculture vs coculture with S. mitis aerobically on BHI-YE HP at 37.C and 5% CO2

Organism:

Streptococcus mitis; Haemophilus parainfluenzae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29208 GPL29207

4 Samples

Download data: CSV, FA, FAA, GFF

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain 723 strains expressing methyltransferase ModA2 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19560

6 Samples

Download data: TXT

(Submitter supplied) We used an in vitro evolved strain of nontypeable Haemophilus influenzae (NTHI) to examine the metabolic contributions to persistence RNASeq analysis identified 55 transcripts that significantly changed in amount within in vitro biofilms formed by RM33, as compared to the parental strain. Expression of genes encoding all enzymes within the tryptophan and glycogen pathways was significantly increased in biofilms formed by RM33 and the parental strains. more...

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19560

8 Samples

Download data: TXT

(Submitter supplied) Haemophilus influenzae responds to the steroid beclomethasone used in asthma therapy by modulation of biofilm formation and antibiotic tolerance

Organism:

Haemophilus influenzae Rd KW20

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26079

12 Samples

Download data: XLSX

(Submitter supplied) A major gap in understanding infectious diseases is the lack of information about molecular interaction networks between pathogens and the human host. Haemophilus ducreyi causes the genital ulcer disease chancroid in adults and is a leading cause of cutaneous ulcers in children in the tropics. We developed a model in which human volunteers are infected on the upper arm with H. ducreyi until they develop pustules. more...

Organism:

[Haemophilus] ducreyi; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL26639 GPL26640

12 Samples

Download data: TXT

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain R2866 expressing methyltransferase ModA10 (ON) or not (LOCK_OFF).

Organism:

Haemophilus influenzae R2866

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26504

6 Samples

Download data: TXT

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain 84P36H1 strains expressing methyltransferase ModA18 (ON) or not (OFF).

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain 10P129H1 strains expressing methyltransferase ModA15 (ON) or not (OFF).

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) comparison of RNA expression profiles from NTHi strain 1209 strains expressing methyltransferase ModA9 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) comparison of RNA expression profiles from NTHi strain 477 strains expressing methyltransferase ModA5 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) comparison of RNA expression profiles from NTHi strain C486 strains expressing methyltransferase ModA4 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) To potentially understand how H. ducreyi responds to membrane stress, here we defined RpoE-dependent genes using RNA-Seq. We identified 180 RpoE-dependent genes, of which 98% were upregulated; a major set of these genes encodes homologues of envelope maintenance and repair factors. We also identified and validated a putative RpoE promoter consensus sequence, which was enriched in the majority of RpoE-dependent targets. more...

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21166

24 Samples

Download data: XLSX

(Submitter supplied) To better understand the role of H. ducreyi CpxRA in controlling virulence determinants, here we defined genes potentially regulated by CpxRA by using RNA-Seq. Activation of CpxR by deletion of cpxA repressed nearly 70% of its targets, including seven established virulence determinants. Inactivation of CpxR by deletion of cpxR differentially regulated few genes and increased the expression of one virulence determinant. more...

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21166

36 Samples

Download data: XLSX

(Submitter supplied) To better understand the molecular mechanisms underlying Haemophilus ducreyi infection in humans, here we determined the transcriptional profile of H. ducreyi in human lesions using RNA-Seq and compared it to that of in vitro growth. We were able to show that the in vivo transcriptome did not resemble that of in vitro growth. Compared to the inoculum, H. ducreyi harvested from pustules differentially expressed ~120 genes, of which 68 were upregulated. more...

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21166

4 Samples

Download data: XLSX

(Submitter supplied) Characterization of host-pathogen interactions is critical for the development of next-generation therapies and vaccines. Classical approaches involve the use of transformed cell lines and/or animal models which may not reflect the complexity and response of the human host. We reconstituted the ciliated human bronchial epithelium in vitro using primary bronchial epithelial cells to simultaneously monitor the infection-linked global changes in nontypeable Haemophilus influenzae (NTHi) and infected host epithelia gene expression by dual RNA-seq. more...

Organism:

Haemophilus influenzae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL19560

24 Samples

Download data: TXT