GEO DataSets

(Submitter supplied) RNA-seq on total RNA of wild type (WT) and set2 mutant (set2d). To determine the changes in transcriptome profile when set2 gene is delete in comparision to WT cells.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13988

4 Samples

Download data: TXT

(Submitter supplied) We characterized fission yeast cellular responses to a novel stress inducer, non-thermal atmospheric-pressure plasma.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13988

15 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL13988 GPL20584

24 Samples

Download data: BW

(Submitter supplied) Investigate the role of topoisomerase 1 in the response to oxidative stress

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13988

8 Samples

Download data: TXT

(Submitter supplied) We compare mettl4 KO and WT samples from different species to understand how mettl4 affects the alterative splicing

Organism:

Schizosaccharomyces pombe; Danio rerio; Drosophila melanogaster; Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

5 related Platforms

23 Samples

Download data: MATRIX

(Submitter supplied) Here, we used RNA-seq to quantify expression conservation across many Ascomycete yeasts.

Organism:

Lachancea waltii; Saccharomyces cerevisiae; Candida albicans; Schizosaccharomyces japonicus; Schizosaccharomyces octosporus; Saccharomyces bayanus; Yarrowia lipolytica; Naumovozyma castellii; Schizosaccharomyces pombe; Nakaseomyces glabratus; Kluyveromyces lactis

Type:

Expression profiling by high throughput sequencing

11 related Platforms

11 Samples

Download data: TXT

(Submitter supplied) Heterochromatin formation requires several steps involving: nucleation followed by spreading of large, silent domains and its faithful re-establishment and maintenance after each replication cycle. The essential histone chaperone FACT has been implicated in heterochromatin silencing, however, little is known about the specificity of FACT in this process. Here, by applying single cell assays, ChIP and genetics, we show that FACT mutants have defects in the spreading of heterochromatin at subtelomeres and mating type locus in fission yeast. more...

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13988

8 Samples

Download data: BW

(Submitter supplied) Prp2 (U2AF65) is an essential splicing factor, that recognizes the poly-Py track near the 3' SS. We have previously observed that when Prp2 is inactivated using a prp2 temperature-sensitive allele, while most of the pre-mRNA is not processed, some are normally spliced. This observation even more noticeable during meiosis, since pre-mRNA regulation is executed at different levels (splicing, polyadenylation and stability). more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13988

2 Samples

Download data: XLSX

(Submitter supplied) RNA-sequencing (RNA-Seq) protocols and bioinformatic pipelines are designed to streamline downstream analyses on sequences believed to be the most important. Here, we have challenged this dogma by preserving ribosomal RNA (rRNA) in our samples and by lowering the minimal RNA size window of our small RNA-Seq analyses to 8 nt

Organism:

Drosophila melanogaster; Schizosaccharomyces pombe; Saccharomyces cerevisiae; Arabidopsis thaliana; Homo sapiens; Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

6 related Platforms

11 Samples

Download data: FA

(Submitter supplied) Gene expression profiles in Schizosaccharomyces pombe mid-log-phase cells grown in three different Synthetic Dextrose medium with different glucose content were compared using the RNA sequencing method. Expression patterns of genes that function in carbohydrate metabolism were downregulated as expected, and these genes were downregulated in line with the increase in glucose content. Significant and consistent changes in the expression were observed such as genes that encoding retrotransposable elements, heat shock proteins, glutathione S-transferase, cell agglutination protein, and conserved fungal proteins.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13988

3 Samples

Download data: XLSX

(Submitter supplied) This RNA-Seq analysis compares gene expression of the RNA polymerase II CTD-mutants contrasting to the WT RNA polymerase II CTD (with isogenic 3' selection marker) in the fission yeast S. pombe

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13988 GPL28961

29 Samples

Download data: TXT, XLSX

(Submitter supplied) Genes embedded in H3 lysine 9 methylation (H3K9me)-dependent heterochromatin are transcriptionally silenced. In fission yeast, Schizosaccharomyces pombe, H3K9me-mediated heterochromatin silencing can be transmitted through cell division provided the counteracting demethylase Epe1 is absent. It is possible that under certain conditions wild-type cells might utilize heterochromatin heritability to form epimutations, phenotypes mediated by unstable silencing rather than DNA changes. more...

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

4 related Platforms

49 Samples

Download data: BW, CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae; Kluyveromyces lactis; Nakaseomyces glabratus; Naumovozyma castellii; Saccharomyces paradoxus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

10 related Platforms

128 Samples

Download data: BW

(Submitter supplied) We measured global expression profiles in SFP1 deletion mutants and isogenic wild type strains during lag and log phase growth. To match the physiology of each species as well as minimize the effect on gene expression from differences in early growth rates, we profiled expression during log growth and lag after glucose repletion as previously described (Thompson et al., 2013).

Organism:

Saccharomyces cerevisiae; Kluyveromyces lactis; Schizosaccharomyces pombe; Nakaseomyces glabratus; Naumovozyma castellii; Saccharomyces paradoxus

Type:

Expression profiling by high throughput sequencing

6 related Platforms

58 Samples

Download data: TXT

(Submitter supplied) Histone H2B mono-ubiquitylation (H2Bub1) and phosphorylation of elongation factor Spt5 by cyclin-dependent kinase 9 (Cdk9) occur during transcription by RNA polymerase II (RNAPII), and are mutually dependent in fission yeast. How Cdk9 activity and H2Bub1 cooperate to regulate the expression of individual genes remains unclear. Here we show Cdk9 inhibition or H2Bub1 loss induces intragenic antisense transcription of distinct gene subsets; ablation of both pathways derepresses antisense transcription of over half the genome. more...

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL20584 GPL13988

24 Samples

Download data: BIGWIG

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by genome tiling array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL13988 GPL7715

20 Samples

Download data: BEDGRAPH, CEL

(Submitter supplied) Regulation of heterochromatin is critical for genome stability. Different states of methylated H3K9 have been discovered with distinct roles in heterochromatin formation and silencing. However, the control of the transition from H3K9me2 to H3K9me3 is still unclear. Here we investigate the role of the conserved bromodomain AAA-ATPase, Abo1, involved in maintaining the global nucleosome organization in fission yeast. more...

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13988

8 Samples

Download data: BEDGRAPH

(Submitter supplied) RNA sequencing of total RNA purified from wild type S. pombe cells after 0, 5 and 24 hours culture in YES containing 100% D2O (deuterium oxide).

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13988

12 Samples

Download data: TXT

(Submitter supplied) The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA Polymerase II passage through chromatin. It remains unknown to what extent they cooperate in vivo. Here, we used genome-wide, genetic, biochemical and microscopic approaches to dissect their interplay in the fission yeast Schizosaccharomyces pombe. We show that FACT and H2Bub repress antisense transcripts near the 5’ end and inside gene bodies, respectively. more...

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13988

44 Samples

Download data: BEDGRAPH

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13988

84 Samples

Download data: BEDGRAPH, BW