GEO DataSets

(Submitter supplied) Aedes aegypti mosquito, vector for several viral diseases, undergoes significant physiological changes after a blood meal. Through single-cell RNA sequencing and metabolomics, we unveiled dynamic cellular composition and metabolic adaptations within abdominal midgut and fat body tissues. We revealed high cell diversity, specialized in digestion, metabolism, immunity, and reproduction. While the midgut primarily comprises enterocytes, enteroendocrine, cardia and intestinal stem cells, the fat body consists of not only trophocytes and oenocytes, but also a substantial population of hemocytes and fat body-yolk cells (FYC). more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

4 Samples

Download data: MTX, TSV

(Submitter supplied) Chromosomal inversions play a fundamental role in evolution and have been shown to be responsible for the epidemiologically important traits in malaria mosquitoes. However, they have never been characterized in the major vector of arboviruses Aedes aegypti because of the poor structure of its polytene chromosomes. In this study, we applied a Hi-C proximity ligation approach to identify chromosomal inversions in 23 recently collected strains of Ae. more...

Organism:

Aedes mascarensis; Aedes aegypti

Type:

Other

Platforms:

GPL26709 GPL28657 GPL33756

26 Samples

Download data: HIC, TXT

(Submitter supplied) Understanding the molecular determinants of the mosquitoes’ competence to host the viruses holds promise to prevent their transmission. We performed transcriptomic analysis with data obtained from RNA-seq of dengue- or naïve- infected mosquitoes on day 1, 2, and 7 post infections

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

18 Samples

Download data: XLSX

(Submitter supplied) Wolbachia pipientis is an intracellular symbiotic bacterium found in insects and arthropods. Wolbachia can decrease the vectorial capacity for various pathogens, such as the dengue virus, in Aedes aegypti. The purpose of this study was to determine the effect of Wolbachia (wMel strain) on the vectorial capacity of Ae. aegypti for Dirofilaria immitis. We analyzed gene expression patterns by RNA-seq in addition to the D. more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

4 Samples

Download data: XLSX

(Submitter supplied) Midgut is an important immune organ of Aedes aegypti. To help further studies on host-pathogen interaction of midgut, we established a midgut cell atlas using scRNA-seq.

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

2 Samples

Download data: MTX, TSV

(Submitter supplied) Mosquitoes are the most notorious hematophagous insects and due to their blood feeding behavior and genetic compatibility, numerous mosquito species are highly efficient vectors for certain human pathogenic parasites and viruses. The mosquito midgut is the principal organ of blood meal digestion and nutrient absorption. It is also the initial site of infection with blood meal acquired parasites and viruses. more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

2 Samples

Download data: MTX, TSV

(Submitter supplied) This study explores the impact of disrupting the circadian clock through a Cycle gene knockout (KO) on the transcriptome of Aedes aegypti mosquitoes. The investigation aims to uncover the resulting alterations in gene expression patterns and physiological processes. Transcriptome analysis was conducted on Cyc knockout (AeCyc-/-) and wild-type mosquitoes at four time points in a light-dark cycle. The study identified system-driven genes that exhibit rhythmic expression independently of the core clock machinery. more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

48 Samples

Download data: TXT

(Submitter supplied) Female Aedes aegypti mosquitoes impose a severe global public health burden as primary vectors of multiple viral and parasitic pathogens. Under optimal environmental conditions, Aedes aegypti females have access to human hosts that provide blood proteins for egg development, conspecific males that provide sperm for fertilization, and freshwater that serves as an egg-laying substrate suitable for offspring survival. more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

33 Samples

Download data: TXT

(Submitter supplied) In this study, 10x Chromium technology was applied to quantify transcripts from single-cell nuclei of adult male and female brain of Aedes aegypti, a medically important mosquito vector that transmits yellow fever, dengue, chikungunya, and Zika viruses to humans.

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

2 Samples

Download data: MTX, TSV

(Submitter supplied) snRNA-seq was used to detect the expression of different chemoreciptors in the antenna and maxillary palp nuclei. Using snRNA-seq, we confirm the coexpression of different cheomreceptos in the same nuclei.

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

3 Samples

Download data: H5

(Submitter supplied) We report here RNAseq analysis of the comparison of gene expression between sugar fed and blood fed Aedes aegypti ovaries, to understand blood meal induced changes in gene expression.

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28657

19 Samples

Download data: TXT