GEO DataSets

(Submitter supplied) Entamoeba histolytica trophozoites epigenetically silenced for ameobapore A (G3 parasites) were additionally silenced for the Rhomboid gene Rom1 (EHI_197460) using the mechanism outlined in Mirelman et al. Parasite. 2008 Sep;15(3):266-74 (PMID 18814693). Rom1 silencing was confirmed by RT-PCR. Gene expression in these parasites was compared to that of the parent G3 strain.

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

4 Samples

Download data: CEL

(Submitter supplied) A myb domain containing protein of the SHAQKY family, EhMyb-dr, was overexpressed in E. histolytica trophozoites, and effects on transcription were measured.

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

9 Samples

Download data: CEL

(Submitter supplied) The unicellular eukaryote Entamoeba histolytica is a human parasite that causes amebic dysentery and liver abscess. A genome-wide analysis of gene expression modulated by intestinal colonization and invasion identified an up-regulated transcript that encoded a putative high-mobility-group box (HMGB) protein, EhHMGB1. We tested if EhHMGB1 encoded a functional HMGB protein, and determined its role in control of parasite gene expression. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

6 Samples

Download data: CEL

(Submitter supplied) The E. histolytica transcription factor URE3-BP is a calcium-responsive regulator of two E. histolytica virulence genes, hgl5 and fdx1. URE3-BP was previously identified by a yeast one-hybrid screen of E. histolytica proteins capable of binding to the sequence TATTCTATT (URE3) in the promoter regions of hgl5 and fdx1. In this work precise definition of the consensus URE3 element was performed by electrophoretic mobility shift assays (EMSA) using base-substituted oligonucleotides, and the consensus motif validated using episomal reporter constructs. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

11 Samples

Download data: CEL

(Submitter supplied) E. histolytica of strains HM1:IMSS (virulent) and Rahman (avirulent) were examined for response to oxidative and nitrosative stress.

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

15 Samples

Download data: CEL

(Submitter supplied) In order to dtermine the role of histone actylation in gene expresssion, E. histolytica 200:NIH trophozoites were treated with the HDAC inhibitor Trichostatin A, or grown in short chain fatty acids, which have been shown to alter histone actylation patterns in Entamoeba species. Keywords: Expression

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

8 Samples

Download data: CEL

(Submitter supplied) A genome-wide transcriptional analysis of Entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture. Adaptation to the intestinal environment was accompanied by increases in a subset of cell signaling genes including transmembrane kinases, ras and rho family GTPases, and calcium binding proteins. Significant decreases in mRNA abundance for genes involved in glycolysis and concomitant increases in lipases were consistent with a change in energy metabolism. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

7 Samples

Download data: CEL

(Submitter supplied) We examined the stress response in Entamoeba histolytica trophozoites by comparing untreated log-phase HM-1:IMSS trophozoites to those subjected to heat shock at 42C for 1 hour. Keywords: stress reponse

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

4 Samples

Download data: CEL

(Submitter supplied) We compared multiple strains of lab trophozoites to recent clinical isolates. Clinical isolates were grown in xenic media, and maintained many characteristics of the cyst stage of devlopment Keywords: Stage conversion

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

19 Samples

Download data: CEL

(Submitter supplied) Expression profiling of treatment of E. histolytica HM-1:IMSS trophozoites with 5-Aza cytidine, an inhibitor of DNA methyltransferase Keywords: drug treatment

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

4 Samples

Download data: CEL