GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by genome tiling array; Genome binding/occupancy profiling by genome tiling array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9377 GPL7070

185 Samples

Download data: BAR, CEL, WIG

(Submitter supplied) Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain to be identified. We profiled a compendium of 50 yeast strains carrying conditional alleles or complete deletions of genes involved in transcriptional regulation, histone biology and chromatin remodeling, as well as compounds that target transcription and histone deacetylases, to assess their respective roles in nucleosome positioning and transcription. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL7070

107 Samples

Download data: BAR, CEL

(Submitter supplied) Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain to be identified. We profiled a compendium of 50 yeast strains carrying conditional alleles or complete deletions of genes involved in transcriptional regulation, histone biology and chromatin remodeling, as well as compounds that target transcription and histone deacetylases, to assess their respective roles in nucleosome positioning and transcription. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by genome tiling array

Platform:

GPL7070

62 Samples

Download data: BAR, CEL

(Submitter supplied) We grew wild type and corresponding mutant abf1 and rap1 temperature sensitive yeast at 25 C, then for one hour after shifting to 37 C. We then prepared nucleosomal (mostly mononucleosomal) DNA and hybridized to tiling arrays.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL7070 GPL7249

5 Samples

Download data: BAR, CEL, TXT

(Submitter supplied) It is currently believed that termination by RNAPII occurs differently depending whether a transcript contains or lacks a polyadenylation signal (PAS). By screening for factors deficient for PAS-dependent termination in an in vivo reporter assay, we identified Sen1, a putative helicase mainly known for its role in PAS-independent termination of snoRNAs. We show for the first time that Sen1 regulates transcription termination at protein-encoding genes genome-wide. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by genome tiling array; Genome binding/occupancy profiling by genome tiling array

Platform:

GPL7070

7 Samples

Download data: BAR, CEL

(Submitter supplied) Cwc21 (complexed with CEF1 protein 21) is a 135 amino acid yeast protein that shares homology with the N-terminal domain of human SRm300, a large serine/arginine-repeat protein shown previously to associate with the splicing coactivator SRm160. Proteomic analysis of spliceosomal complexes has suggested a role for Cwc21 and SRm300 at the core of the spliceosome. However, specific functions for these proteins have remained elusive. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by genome tiling array

Platform:

GPL7070

5 Samples

Download data: BAR, CEL

(Submitter supplied) A two-colour cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression. We describe a fluorescent reporter system that exploits the functional genomic tools available in budding yeast to systematically assess consequences of genetic perturbations on gene expression. We used our Reporter-Synthetic Genetic Array (R-SGA) method to screen for regulators of core histone gene expression. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL7070

5 Samples

Download data: BAR, CEL

(Submitter supplied) We screened for histone H3 mutations that cause derepression of the uninduced CHA1 promoter and identified six mutants, including one, H3(A111G), that causes a constitutively active chromatin configuratin at the uninduced CHA1 promoter. We examined genomewide nucleosome occupancy in the A111G mutant and compared it to wild type yeast; that is the data deposited here. Keywords: Nucleosomal DNA from wild type and histone H3(A111G) mutant yeast

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL7070 GPL7250

8 Samples

Download data: BAR, CEL, GRD

(Submitter supplied) The platform contains 25 nucleotide probes spaced every 8 bp covering one strand of the whole /Saccharomyces cerevisiae/ genome sequence and a second set of probes offset 4 bp from the first set to cover the other strand. Lee et al. (2007) Nature Genetics 39: 1235-1244. Protocol: see manufacturer's web site

Organism:

Saccharomyces cerevisiae

8 Series

190 Samples

Download data: TXT