GEO DataSets

(Submitter supplied) We developed a novel approach to isolate tumor cells with high purity from bone marrow which was subjected to immunomagnetic enrichment using EpCAM beads followed by fluorescence activated cell sorting (IE/FACS) to isolate EpCAM-positive cells away from leukocytes (CD45+). For RNA profiling, QPCR analysis was performed on sixty four (64) cancer-related genes using Taqman® low density arrays. For non-tumor controls, RNA profiling was performed on matched leukocytes (CD45+) isolated from the same enriched bone marrow samples from 17 of the 30 patients.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL16020

45 Samples

Download data: TXT

(Submitter supplied) We selected 64 genes from previously published data focused on QPCR analyses of CTCs. Included in the list are clinically relevant genes, stem cell and epithelial-mesenchymal transition (EMT) markers, and highly expressed genes in breast and prostate cancers. We also included EPCAM and PTPRC (encodes CD45) to measure the expression of these markers specific for epithelial cells and leukocytes, respectively. more...

Organism:

Homo sapiens

3 Series

272 Samples

Download data