GEO DataSets

(Submitter supplied) Given that transition metals are essential cofactors in central biological processes, misallocation of the wrong metal ion to a metalloprotein can have resounding and often detrimental effects on diverse aspects of cellular physiology. Therefore, in an attempt to characterize unique and shared responses to chemically similar metals we have reconstructed physiological behaviors of Halobacterium NRC-1, an archaeal halophile, in sub-lethal levels of Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II). more...

Organism:

Halobacterium salinarum NRC-1

Type:

Expression profiling by array

Platform:

GPL3739

8 Samples

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(Submitter supplied) MIAME platform type: spotted MIAME availability: Arrays printed at ISB can be purchased, contact [email protected] for information. Standard Operating Procedures for the Virtek(ESI) Spotter *** Always remember that spotter pins are extremely fragile at the tips *** To make spotter source plates: 1. Total volume should be 4-8 ul 2. Usually, DNA should be in 3X SSC and 1.5M betaine. This can be done most accurately be putting an appropriate amount of source material in spotting plates, then drying down with the speedvac and resuspending in the final volume of 3X SSC, 1.5M betaine. more...

Organism:

Halobacterium salinarum NRC-1

70 Series

2 Related Platforms

1747 Samples

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