GSM101390[Accession] - GEO DataSets

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Items: 3

Select item 2000045191.

Effect of gas-substrate limitation on barophilic growth of Methanocaldocccus jannaschii at 500 atm.

(Submitter supplied) Barophilic growth of the hyperthermophilic methanarchaeon Methanocaldococcus jannaschii occurred when gas-substrate availability did not limit growth. In contrast, when growth was limited by gas transfer, no enhancement of growth was evident and a stress response was exhibited at both high and low pressure. A pressure-induced transcriptional response was evident, regardless of whether growth was enhanced by pressure. more...

Organism:: Methanocaldococcus jannaschii

Type:: Expression profiling by array

Platform:: GPL3565
13 Samples

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Accession:: GSE4519

ID:: 200004519

Select item 1000035652.

UCB Methanocaldococcus jannaschii 7K v1.0

(Submitter supplied) Each sub-array contains blocks arranged 4 by 4 with each block running 22 rows by 20 columns. Protocol: Primers (Invitrogen) were designed for all 1738 open reading frames (ORFs) using Primer 3 software. Polymerase chain reaction was performed with 50 ng of genomic DNA, 0.8 uM of each forward and reverse primer, and 10 U of Taq DNA polymerase (New England Biolabs). Products were precipitated on 96 well plates with isopropanol and 3M sodium acetate at 4C and analyzed on a 0.8% agarose gel. more...