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Items: 4

1.

Identification of chondrocyte subsets during human development

(Submitter supplied) Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD146low/negCD166low/negCD73+CD44lowBMPR1B+) distinguishing the earliest cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development; pellet assays confirmed these cells as functional, chondrocyte-restricted progenitors. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Datasets:
GDS5045 GDS5046
Platform:
GPL570
15 Samples
Download data: CEL
Series
Accession:
GSE51812
ID:
200051812
2.
Full record GDS5045

Prechondrocytes

Analysis of LCM-isolated prechondrocytes in chondrogenic condensations and total limb cells at week 6 of embryogenesis. Results provide insight into molecular mechanisms distinguishing prechondrocytes from other cell lineages in developing limbs.
Organism:
Homo sapiens
Type:
Expression profiling by array, transformed count, 2 cell type sets
Platform:
GPL570
Series:
GSE51812
12 Samples
Download data: CEL
3.

[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 06, 2012: annotation table updated with netaffx build 32 June 23, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. more...
Organism:
Homo sapiens
602 DataSets
5670 Series
61 Related Platforms
175576 Samples
Download data
Platform
Accession:
GPL570
ID:
100000570
4.

Remaining limb cells_6wks_LCM_rep4

Organism:
Homo sapiens
Source name:
Human fetal limb 6 wks
Platform:
GPL570
Series:
GSE51812
Dataset:
GDS5045
Download data: CEL
Sample
Accession:
GSM1253165
ID:
301253165
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