GEO DataSets

(Submitter supplied) To survive a dynamic host environment, Mycobacterium tuberculosis must endure a series of challenges from reactive oxygen and nitrogen stress, to drastic shifts in oxygen availability. The mycobacterial Lsr2 protein has been implicated in reactive oxygen defense via direct protection of DNA. To examine the role of Lsr2 in pathogenesis and physiology of M. tuberculosis, we generated a strain deleted for lsr2. more...

Organism:

Mycobacterium tuberculosis H37Rv; Mycobacterium tuberculosis CDC1551; Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL15398

16 Samples

Download data: GPR

(Submitter supplied) 1. Oligos are arrayed in Whatman 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo. 2. Oligos are suspended in 3xSSC to 20 uM and centrifuged at 500 rpm for 2 minutes. 3. Plates are stored at 4oC overnight. 4. Incubate at RT for 30 minutes and shakern for 5 min to aid resuspension of DNA. 5. Centrifuge plates at 500 rpm for 2 minutes. 6. Remove seals 7. Print 8. After completion of print, allow slides to dry through passive evaporation in a protected environment.

Organism:

Mycobacterium tuberculosis CDC1551; Mycobacterium tuberculosis H37Rv

3 Series

33 Samples

Download data