GEO DataSets

(Submitter supplied) MC3T3 cells clone 1b were grown in alpha-MEM with 10% fetal calf serum (FCS, Gibco), 1% penicilin/streptomycin (Pen/Strep) and 1% L-Glutamine (L-Glu). For differentiation experiment, cells were seeded on 6 cm dishes (3x105cells/dish in 5 ml medium) and grownt to confluence for 3 days at 37C / 5% CO2. Cells were then stimulated with osteogenic stimulus (10 mM beta-glycerophosphate (GP, Sigma), 50 µM ascorbic acid (AA, Wako) and 1 mg/ml BMP-2 (Novartis)), or with 10mM GP alone for 1 or 3 days. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS679

Platform:

GPL81

15 Samples

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Response of calvarial MC3T3-1b cells to osteogenic stimulus (beta-glycerophosphate (GP), ascorbic acid (AA) and bone morphogenic protein 2 (BMP-2)) for 0, 1 and 3 days. Notch, Wnt and TGF-β signaling pathways are activated in osteogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 growth protocol, 3 time sets

Platform:

GPL81

Series:

GSE1131

15 Samples

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(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 07, 2012: annotation table updated with netaffx build 32 June 24, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site The MG-U74 set includes 3 arrays with a total of 36899 entries and was indexed 29-Jan-2002. more...

Organism:

Mus musculus

264 DataSets

537 Series

1 Related Platform

6909 Samples

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