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Items: 3

1.

Desiccation and control transcriptomes of Rhodococcus jostii RHA1

(Submitter supplied) Here we report the first transcriptomic analysis of a Gram-positive bacterium to desiccation. Filtered RHA1 cells incubated at either low relative humidity (20%), as an air drying treatment, or high relative humidity (100%), as a control, were transcriptionally profiled over a comprehensive time series. Keywords: stress response time course
Organism:
Rhodococcus jostii RHA1
Type:
Expression profiling by array
Platforms:
GPL6703 GPL3918
42 Samples
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Series
Accession:
GSE10378
ID:
200010378
2.

MERH03-S1-0003 (Rhodococcus sp. RHA1 11K)

(Submitter supplied) Oligonucleotides are consolidated into 384-well plates, lyophilized by speed-Vac, and resuspended in 3x SSC to a printing concentration of 30uM. Oligos are printed on aminosilane slides (Erie, Hudson, NH, USA) with a Biorobotics Microgrid II microarray printer (Genomic Solutions). Microspot 10K quill pins (Biorobotics) in a 48-pin tool were used to deposit <0.5 nL per spot onto the slide. The resulting microarrays have a 4 x12 subgrid layout with 392 spots per subgrid, each spot having approximate diameter and pitch of 90 µm and 190µm, respectively. more...
Organism:
Rhodococcus jostii RHA1
1 Series
21 Samples
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Platform
Accession:
GPL6703
ID:
100006703
3.

Rhodococcus jostii RHA1 incubated at 100% relative humidity for 1 h, replicate 2

Organism:
Rhodococcus jostii RHA1
Source name:
mid-exponential phase, benzoate-grown Rhodococcus jostii RHA1 incubated at 100% relative humidity (30°C) for 1 h (channel 1) mid-exponential phase, benzoate-grown Rhodococcus jostii RHA1 at time zero (just after filtration onto nitrocellulose membrane) (channel 2)
Platform:
GPL6703
Series:
GSE10378
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Sample
Accession:
GSM282365
ID:
300282365
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Supplemental Content

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