GSM3083020[Accession] - GEO DataSets

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Items: 4

Select item 2001127591.

Parallel DNA and RNA profiling of EpCAM-positive cells in bone marrow and primary tumor tissue with positive disseminated tumor cell count via immunomagnetic Enrichment/Flow Cytometry of metastatic breast cancer patients confirm their malignant nature

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by RT-PCR; Genome variation profiling by array

Platforms:: GPL6359 GPL16020
106 Samples

Download data: TXT

Series

Accession:: GSE112759

ID:: 200112759

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001127572.

RNA profiling of EpCAM-positive cells in bone marrow of early breast cancer (EBC) patients

(Submitter supplied) We developed a novel approach to isolate tumor cells with high purity from bone marrow which was subjected to immunomagnetic enrichment using EpCAM beads followed by fluorescence activated cell sorting (IE/FACS) to isolate EpCAM-positive cells away from leukocytes (CD45+). For RNA profiling, QPCR analysis was performed on sixty four (64) cancer-related genes using Taqman® low density arrays. For non-tumor controls, RNA profiling was performed on matched leukocytes (CD45+) isolated from the same enriched bone marrow samples from 17 of the 30 patients.

Organism:: Homo sapiens

Type:: Expression profiling by RT-PCR

Platform:: GPL16020
45 Samples

Download data: TXT

Series

Accession:: GSE112757

ID:: 200112757

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000160203.

Applied Biosystems TaqMan TLDA 64-Breast cancer associated-gene panel

(Submitter supplied) We selected 64 genes from previously published data focused on QPCR analyses of CTCs. Included in the list are clinically relevant genes, stem cell and epithelial-mesenchymal transition (EMT) markers, and highly expressed genes in breast and prostate cancers. We also included EPCAM and PTPRC (encodes CD45) to measure the expression of these markers specific for epithelial cells and leukocytes, respectively. more...