GSM366258[Accession] - GEO DataSets

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Items: 3

Select item 2000145341.

Combined genome-wide expression profiling and targeted RNA interference in primary mouse macrophages

(Submitter supplied) Here we have used targeted RNA interference to suppress the expression of a number of key genes associated with IFN signalling in murine macrophages prior to activation. Genome-wide changes in transcript abundance caused by siRNA activity were measured using Affymetrix exon-arrays in the presence or absence of IFNγ stimulation. Transfection of murine bone-marrow derived macrophages (BMDMs) with non-targeting (control) siRNA and 11 sequence-specific siRNAs was performed using a cationic lipid transfection reagent (Lipofectamine2000) prior to stimulation with IFNγ. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6096
90 Samples

Download data: CEL, CHP

Series

Accession:: GSE14534

ID:: 200014534

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000060962.

[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html #%create_date=Wed Mar 11 17:52:08 2009 PDT #%chip_type=MoEx-1_0-st-v1 #%lib_set_name=MoEx-1_0-st #%lib_set_version=v1 #%genome-species=Mus musculus #%genome-version=mm9 #%genome-version-ucsc=mm9 #%genome-version-ncbi=37 #%genome-version-create_date=2007-07 #%netaffx-annotation-date=2009-03-16 #%netaffx-annotation-netaffx-build=28 Protocol: See manufacturer's web site