GEO DataSets

(Submitter supplied) Subspecies of the Atlantic killifish, Fundulus heteroclitus, differ in their maximum thermal tolerance. To determine whether there is a link between the heat shock response (HSR) and maximum thermal tolerance, we exposed 20ºC acclimated killifish from these subspecies to a 2hr heat shock at 34ºC and examined gene expression during heat shock and recovery using real time quantitative PCR and a heterologous cDNA microarray designed for salmonid fishes. more...

Organism:

Oncorhynchus mykiss; Oncorhynchus tshawytscha; Salmo salar; Fundulus heteroclitus; Coregonus clupeaformis; Osmerus mordax

Type:

Expression profiling by array

Platform:

GPL2716

16 Samples

Download data: MEV

(Submitter supplied) The microarrays have a 4-by-12 metagrid layout with 19 X19 spot subgrid, each spot having an approximate diameter and pitch of 100 um and 0.20 mm, respectively. A 280 bp GFP (green fluorescent protein) cDNA was amplified from a GFP clone (Clontech) using the primers (5'-GAAACATTCTTGGACACAAATTGG-3') and (5'-GCAGCTGTTACAAACTCAAGAAGG-3') and printed in each subgrid corner to assist in gridding. Six exogenous genome (Arabidopsis) cDNAs were amplified from the following clones kindly provided by The Arabidopsis Information Resource: rubisco activase [GenBank:T41667], protochlorophyllide reductase precursor [GenBank:R30630], chlorophyll a/b-binding protein CP29 [GenBank:N65746], PSII oxygen-evolving complex protein 2 [GenBank:H36167], tonoplast intrinsic protein root-specific RB7 [GenBank:AA067532] and ferredoxin (2Fe_2S) precursor [GenBank:W43249] and spotted in quadruplicate on each microarray. more...

Organism:

Osmerus mordax; Oncorhynchus mykiss; Salmo salar; Coregonus clupeaformis; Oncorhynchus tshawytscha

33 Series

1 Related Platform

1235 Samples

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