GSM5211786[Accession] - GEO DataSets

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Items: 4

Select item 2001681071.

Generation and Characterization of Stable Pig Pre-gastrulation Epiblast Stem Cell Lines

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Sus scrofa

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:: GPL22918 GPL22475 GPL26285
68 Samples

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Accession:: GSE168107

ID:: 200168107

Select item 2001681042.

Generation and Characterization of Stable Pig Pre-gastrulation Epiblast Stem Cell Lines [HiC]

(Submitter supplied) Despite ongoing attempts since the 1990s, no stable embryonic stem cell line has been established in pigs. Here, guided by insights from large-scale single-cell RNA-sequencing profiling of pig embryos from embryonic day (E) 0 to E14, we developed an in vitro culture system for establishing and maintaining stable pluripotent stem cell lines from pig E10 pre-gastrulation epiblasts (pgEpiSCs). Enabled by chemical inhibition of WNT-related signaling in culture, the pgEpiSCs maintain their pluripotency and normal karyotypes after more than 200 passages. more...