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Items: 3

1.

Improved prediction of bacterial CRISPRi guide efficiency from depletion screens through mixed-effect machine learning and data integration

(Submitter supplied) CRISPR interference (CRISPRi), the targeting of a catalytically dead Cas protein to block transcription, is the leading technique to silence gene expression in bacteria. However, design rules for CRISPRi remain poorly defined, limiting predictable design for gene interrogation, pathway manipulation, and high-throughput screens. Here we develop a best-in-class prediction algorithm for guide silencing efficiency by systematically investigating factors influencing guide depletion in multiple genome-wide essentiality screens, with the surprising discovery that gene-specific features such as transcriptional activity substantially impact prediction of guide activity. more...
Organism:
Escherichia coli
Type:
Other
Platform:
GPL25368
8 Samples
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Series
Accession:
GSE196911
ID:
200196911
2.

Illumina NovaSeq 6000 (Escherichia coli)

Organism:
Escherichia coli
72 Series
1000 Samples
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Platform
Accession:
GPL25368
ID:
100025368
3.

Input_2

Organism:
Escherichia coli
Source name:
Escherichia coli MG1655
Platform:
GPL25368
Series:
GSE196911
Download data
Sample
Accession:
GSM5904625
ID:
305904625
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db=gds|term=GSM5904625[Accession]|query=1|qty=2|blobid=MCID_674c349996d9ad0406218d2f|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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