GEO DataSets

(Submitter supplied) Mouse embryonic fibroblasts (MEFs) were generated from 13.5-day-old embryos obtained from heterozygous PKBa mice intercrosses (Yang et al., 2003). Briefly, after dissection of head and visceral organs for genotyping, embryos were minced and trypsinized for 30 min at 37°C. Embryonic fibroblasts were then plated and maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% foetal calf serum (FCS) (Life Technologies), 100 units/ml of penicillin and 100 mg/ml of streptomycin at 37°C in an atmosphere of 5% CO2. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1784

Platform:

GPL339

36 Samples

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Analysis of protein kinase B alpha (PKBalpha) knockout embryonic fibroblasts (MEFs) following treatment with a standard induction cocktail to induce differentiation into adipocytes. Cells examined up to 48 hours following treatment. Results provide insight into the role of PKBalpha in adipogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 5 time sets

Platform:

GPL339

Series:

GSE2746

36 Samples

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(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html The GeneChip Mouse Genome 430A 2.0 Array is accessioned in GEO as GPL8321. June 03, 2009: annotation table updated with netaffx build 28 June 07, 2012: annotation table updated with netaffx build 32 June 24, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site The GeneChip Mouse Genome 430A Array is a single array representing approximately 14,000 well-characterized mouse genes that can be used to explore biology and disease processes. more...

Organism:

Mus musculus

142 DataSets

423 Series

2 Related Platforms

5328 Samples

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