GSM60450[Accession] - GEO DataSets

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Items: 4

Select item 2000027761.

Neurogenic potential of human mesenchymal stem cells revisited

(Submitter supplied) The possibility of generating neural cells from human bone marrow-derived mesenchymal stem cells by simple in vitro treatments is alluring both conceptually and practically. However, whether phenotypic modulations observed after chemical manipulation of such stem cells truly represent a genuine trans-lineage differentiation remains to be established. We have re-evaluated the effects of a frequently reported biochemical approach, based on treatment with butylated hydroxyanisole and dimethylsulphoxide, to bring about such phenotypic conversion by monitoring the morphological changes induced by the treatment in real time, by analysing the expression of phenotype-specific protein markers and by assessing the modulation of the cells’ transcriptome. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS1347
Platform:: GPL2136
11 Samples

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Accession:: GSE2776

ID:: 200002776

Select item 13472.

Neurogenic potential of mesenchymal stem cells

Comparison of mesenchymal stem cells (MSC) treated with dimethylsulphoxide and butylated hydroxyanisole (DMSO/BHA) for 6 or 48 hours to fetal brain (positive control) or adult liver (negative control). Results raise doubts about the DMSO/BHA protocol for generating neural cells from MSCs.

Organism:: Homo sapiens

Type:: Expression profiling by array, log2 ratio, 2 agent, 3 cell type, 3 time sets

Platform:: GPL2136
Series:: GSE2776
11 Samples

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DataSet

Accession:: GDS1347

ID:: 1347

Select item 1000021363.

Micro-CRIBI Human Oligo Array (Operon V2.0)

(Submitter supplied) This set consists of 21,329 70mer oligonucleotides (Operon version 2.0) designed on Human Unigene clusters, mainly in the 3 end terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in two replicates on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. more...