GEO DataSets

(Submitter supplied) We have engineered an RT-active DNA polymerase variant called RT-KTq I614Y that produces error RT‑signatures specific for pseudouridine (Ψ) without prior chemical treatment of the RNA samples. These signatures are amplified during DNA amplicon library preparation and are detected by NGS. This method was applied to distinguish U from Ψ in RNA oligonucleotides (modified or unmodified) used in the previous polymerase screening and oligonucleotides which are designed from the human 18S rRNA at the position around 1445. more...

Organism:

Saccharomyces cerevisiae; synthetic construct

Type:

Other

Platforms:

GPL32628 GPL31112

14 Samples

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