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Items: 3

1.

tRNA structure-seq in peptide-rich droplets

(Submitter supplied) Compartmentalization of RNA in biopolymer-rich membraneless organelles is now understood to be pervasive and critical for the function of extant biology, and has been proposed as a prebiotically-plausible way to accumulate RNA. Compartment-RNA interactions that drive encapsulation have the potential to influence RNA structure and function in compartment- and RNA sequence-dependent ways. Herein, we detail Next-Generation Sequencing (NGS) experiments performed for the first time in membraneless compartments called complex coacervates to characterize the fold of many different transfer RNAs (tRNAs) simultaneously under the potentially denaturing conditions of these compartments. more...
Organism:
synthetic construct; Escherichia coli
Type:
Other
Platforms:
GPL32628 GPL32081
60 Samples
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Series
Accession:
GSE225730
ID:
200225730
2.

NextSeq 2000 (synthetic construct)

Organism:
synthetic construct
12 Series
116 Samples
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Platform
Accession:
GPL32628
ID:
100032628
3.

Yphe control treatment (minus DMS) 10mers / 1:2 / 10 replicate 3

Organism:
synthetic construct
Source name:
T7 transcript of an S. cerevisiae tRNA
Platform:
GPL32628
Series:
GSE225730
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Sample
Accession:
GSM7054855
ID:
307054855
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