GEO DataSets

(Submitter supplied) Two human stromal cell lines, HS-5 and HS-27a, represent functionally distinct components of the bone marrow microenvironment. A third influential component of the microenvironment is resident monocytes and macrophages. The baseline expression profile of normal peripheral blood monocytes was determined and their effect on the gene expression of the stromal cells was investigated in a co-culture sytem. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL44

13 Samples

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(Submitter supplied) The microarrays were constructed using a set of more than 17,000 sequence-verified clones from Research Genetics (Huntsville, AL). Of the 17,761 features (spots) on the microarray, 186 are control nonexpressed or nonhuman sequences or housekeeping genes. UniGene cluster IDs could be assigned by GenBank to 16,592 of the features as of June 1, 2001 (UniGene Build 133), indicating that they are representatives of nonredundant unique genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS12

Platform:

GPL44

8 Samples

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Examination of two functionally distinct human bone marrow stromal cell lines, HS-27a and HS-5. Four independent RNA samples from each cell line analyzed.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 cell line sets

Platform:

GPL44

Series:

GSE463

8 Samples

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(Submitter supplied) Microarray construction and hybridization protocols were modified from DeRisi, J.L., V.R Iyer, and P.O. Brown. Science 278:680-686, 1997. The microarrays were constructed using a set of more than 17000 sequence-verified clones from Research Genetics (UniGene Build 136). The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA).

Organism:

Homo sapiens

2 DataSets

6 Series

39 Samples

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