GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Olea europaea

Type:

Expression profiling by array

Platform:

GPL15288

76 Samples

Download data: GPR

(Submitter supplied) A transcriptomics approach was used as a tool to unravel gene regulatory network underlying salinity response in a salt-tolerant olive cultivar (cv. Kalamon) by simulating as much as possible olive growing conditions in the field. A 135 day long salinity experiment was conducted using one year old trees exposed to NaCl stress for 90 days followed by 45 days of post-stress period. Total RNA was extracted from the root samples after 15, 45 and 90 days of NaCl-treated and un-treated olive trees as well as after 15 and 45 days of post-treatment period and used for microarray hybridizations using a loop design. more...

Organism:

Olea europaea

Type:

Expression profiling by array

Platform:

GPL15288

36 Samples

Download data: GPR

(Submitter supplied) The unique cDNAs were PCR-amplified using Taq polymerase (Minotech, Heraklion, Greece) with the primers (pDNR-F, 2: 5'-CCGCATAACTTCGTATAGC–3' and pDNR-R, 2: 5'-CATGTTCACTTACCTACTGG-3') positioned by the polylinker site. The PCR products were purified using Nucleofast 96 columns (Macherey-Nagel, Duren, Germany). Approximately 3 μg of purified PCR product was placed in 384-well printing plates (Genetix, Hampshire, UK), dried in room temperature and resuspended in printing buffer containing 450 mM NaCl, 45 mM phosphate pH 7.0, 5% [v/v] formamide and 0.01% [v/v] maltoside at a final concentration of 200 μg/μl. more...

Organism:

Olea europaea

3 Series

76 Samples

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